THE EFFECT OF DNA EXTRACTION METHODS ON OBSERVED MICROBIAL COMMUNITIES FROM FIBROUS AND LIQUID RUMEN FRACTIONS OF DAIRY COWS

The Effect of DNA Extraction Methods on Observed Microbial Communities from Fibrous and Liquid Rumen Fractions of Dairy Cows

DNA based methods have been widely used to study the complexity of the rumen microbiota, and it is well known that the method of DNA extraction is a critical step in enabling accurate assessment of this complexity.Rumen fluid (RF) and fibrous content (FC) fractions differ substantially in terms of their physical nature and associated microorganisms

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A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study

Abstract Background Mass spectrometry-based proteomics has reached a stage where it is possible to comprehensively analyze the whole proteome of a cell in one experiment.Here, the employment of stable isotopes has become a standard technique to yield relative abundance values of proteins.In recent times, more and more experiments are conducted that

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THE SCYLLA OF LIBERAL PROGRESSIVISM AND THE CHARYBDIS OF A DOGMATIC VICEGERENT APPROACH IN THE STUDY OF RUSSIAN LITERATURE

This polemical article presents two extreme viewpoints which currently exist in Russian literary criticism and which set the barrier for the Outdoor Television adequate study of Russian literature.While liberal progressivism a priori stands for the negative attitude to Eastern Orthodoxy as Lower Slide Tube such, the uncritical projection of a coher

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Helmet continuous positive airway pressure versus high-flow nasal cannula in COVID-19: a pragmatic randomised clinical trial (COVID HELMET)

Abstract Background Patients with COVID-19 and hypoxaemia despite conventional low-flow oxygen therapy are often treated with high-flow nasal cannula (HFNC) in line with international guidelines.Oxygen delivery by helmet continuous positive airway pressure (CPAP) is a feasible option that enables a higher positive end-expiratory pressure (PEEP) and

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